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Video: Preparation and Use of HIV-1 Infected Primary CD4 T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays
25.9K Views. Rush University Medical Center. Cytotoxicity assays to measure natural killer cell lytic responses to HIV-infected cells is limited by the purity of the target cells. We demonstrate here the isolation of a highly purified population of HIV-1 infected primary T-cell blasts by taking advantage of HIV-1 s ability to down-modulate CD4.
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